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Contents

Slide 3

We create barriers using high levels of HEPA filtration and environmental separation.

We achieve this using different tools and methods. The following includes examples of what we have done in other facilities.

Taconic Farms, NY - World’s largest transgenic mouse breeding facility.

Biotech Research - bioBubble Clean Room in Converted Simple Building

Slide 8

bioBubble Advantages:

Free Standing Positive bioBubble Clean Room

Anatomy of a Free Standing Positive Clean Room

Free Standing Negative Containment Room

Freestanding bioBubble Clean Rooms are available in any size and shape.

Clean Room with Docked Changing Station

Easy, visible access into bioBubble Clean Room through strip door.

Taconic, California - 4-5,000 mice per Clean Room (12 rooms)

It is easier to maintain a clean microcosm when the macrocosm it is housed in is also kept clean.

Slide 18

bioBubble Changing Station

bioBubble Changing Station Specifications.

Changing Station Docked to Positive Clean Room

bioBubble ® Bedding Disposal Unit

bioBubble® Bedding Disposal Unit

bioBubble® Rack Covers

Slide 25

Opaque Transfer Cart in Barrier Hallway

bioBubble® Quarantine Unit

Slide 28

bioBubble® HEPA Vacuum

Slide 30

bioBubble Air Shower

bioBubble Air Shower Specifications

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Dedicated Airlock - Gowning Area

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bioBubble Diffusion Head

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Clean/ Dirty Weak Link

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Supervision

Ease of Supervision - Communicate through vinyl walls without entering the clean room environment.

Improved Social Environment

Caging Options

Positive bioBubble® Clean Room with Negative IVC Rack

Positive IVC Rack in Positive Clean Room

Biotech Research - Using simple static micro-isolator cages.

Taconic, California - Using open wire bar lid cages.

Taconic Farms, NY - World’s largest transgenic mouse breeding facility. Using static micro-isolator caging.

Taconic, NY - CAD Rendering

Architectural Renovations and Surgical Suites

Renovation with Free Standing Positive Airlock

Architectural Renovation with Negative Airlock

Airlock Entry / Exit to Quarantine Room with Clear and Opaque Material

Ohio State University

Ohio State - Architectural Renovation

Air Duct View from the Inside

Architectural Renovation - Rack Enclosures

UCLA - Negative surgical room and prep area for Q Fever sheep research

Children’s Hospital - Cincinnati - Surgical Table

Surgical Suite in Historic Military Building

Texas Tech - Swine Surgical Suite

BC Research - Positive Airlock, Negative Quarantine Area, Positive Procedural Area

BC Research - Positive Airlock

Negative Pressure Area with Quarantine Units

Positive Procedural Area for Embryo Transplants

Facility Design

University of Calgary CAD Drawing for Barrier and Quarantine Area

University of Calgary - Quarantine Room with Airlock Entry

Quarantine Units, Changing Stations, Pass-through

Pass-through from Quarantine Room to Barrier at University of Calgary

University of Calgary Pass-through from Quarantine Room to Barrier Hallway

Barrier Hallway Transfer Cart Air Shower

Changing Station in Barrier Hallway

Air Showers used in Series Barrier Hallway

The Mouse Hotel

Positive Architectural Renovation with negative Airlock - suitable for a “Mouse Hotel”

Quarantine Unit in bioBubble® Clean Room

CAD Drawing of a Transgenic Facility in Tokyo, Japan

Tokyo, Japan - Multiple bioBubble® Clean Rooms

Space conservation using bioBubble technology

Facility Design for Transgenic or Immunocompromised Animals

BSL3 and BSL4

Facilities involved with BSL3 and BSL4 work

Conversion of a Hard Room in a Conventional Facility for BSL3 Tuberculosis Research

BSL3 - TB Research Room - CAD

University of Pittsburgh - BSL3 Primate Research

University of Pittsburgh - CAD

University of Pittsburgh - BSL3

Free Standing Negative Containment Room- BSL3 Poultry Research

Health Canada - BSL4 - Primate/ Rabbit Research

BSL4 - Primate/ Rabbit Research

Bioterrorism Response

Bioterrorism Response Units

CDC - Bioterrorism Response Unit - CAD

CDC - Bioterrorism Response Units - Details

bioBubble Details and Extras

Biological Safety Cabinet Docked to bioBubble Clean Room

Cabinet Docked to bioBubble Clean Room

bioBubble Clean Room Details

Wall Anchor Details

Water Line Detail

Our Architectural Renovations can be adapted to almost any configuration

Ceiling Detail for Architectural Renovation

UCLA - Accordion Door

Negative bioBubble Clean Room in Greenhouse

bioBubble Clean Room in Greenhouse with Airlock

Black Vinyl Renovation- Reverse Light Cycle

Architectural Renovation Using Black Vinyl

Free Standing Black Vinyl Clean Room

Black Vinyl Clean Room - Accordion Partition

Slide 129

Taconic’s West Coast Operations

Husbandry levels improve due to work visibility

Taconic WCO - Using open wire bar lid cages

Slide 133

Wall Anchor Detail - Screws